Authors: Dian Mulawarmanti, Kristanti Parisihni, Widyastuti Widyastuti.
Background: Diabetes and periodontitis affect a large number of populations worldwide. Osteoprogeterin (OPG) is cytokine regulating osteoclastogenesis, related to osteoclast and osteoblast implicated in various inflammations including periodontitis and diabetes. Hyperbaric oxygen therapy (HBOT) has been used as adjuvant therapy to heal chronic wound problem. Sticopus Hermanii (SH) has been consumed as food rich with content of sulfated glycosaminoglycans (GAGs) which affect wound healing.
The aim of this study is to examine the effect of HBOT and Sticopus hermanii to the expression of OPG, osteoclast and osteoblast in periodontitis diabetic rats.
Methods and Material: The research was an experimental laboratory with posttest only control group design. Thirty male Wistar rats aged 8-10 weeks were equally divided in 5 groups of: healthy group (G1), diabetic periodontitis group (G2), diabetic periodontitis group treated SH (G3), diabetic periodontitis group treated with HBOT 2,4 ATA 3x30’ interval 5’ for 7 days (G4), diabetic periodontitis group treated with combination SH-HBOT (G5). Diabetes was conducted by single dose of streptozotocin 65 mg/kg of BW intraperitoneal (diabetes condition if blood glucose levels >230mg /dL), while periodontitis were induced by Porphyromonas gingivalis ATCC 3327.
The OPG expressions were examined by immunohistochemistry, while the number of osteoclast and osteoblast by hematoxylin eosin staining.
Results: It was found that combination SH-HBOT group (G5) in OPG could increase (12.50 ± 2.082) than diabetic-periodontitis group(G2) 2.50 ± 0.577. The combination SH-HBOT group (G5) in osteoblast can increased significantly (28.47 ± 3.20) than diabetic-periodontitis group(G2) 9.22 ± 4.95. Osteoclast was decreased significantly in combination SH-HBOT group (G5) 3.50 ± 0.957 than diabetic-periodontitis group 0.500 ± 816. (p<0.05).
Conclusion: The combination of SH-HBOT increased OPG expression, osteoblast, and reduced the number of osteoclasts in periodontitis diabetic rats.
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