Authors: Rubi G, Muhammad Aslamkhan.
Objectives: To study the susceptible gene in reference to the treatment of relapsers & non-responders various ethnic tribes. To determines the role of IL-10 and IL-28B gene polymorphism in ethnic group of Punjab.
Subjects & Methods: Total of 402 patients of HCV was recruited for this study. Patients diagnosed anti-HCV positive confirmed by HCV PCR qualitative test. All these selected patients diagnosed for HCV PCR (qualitative & quantitative), genotyping. Who have taken the standard treatment of IFN/Ribavirin 20-36 weeks. Objective of the study was to find the role of gene susceptible in responders, relapsers & non-responders of HCV. Study was undertaken to find the role of gene Polymorphism of IL10 and IL28B BstU 1, Rsa and Bst61 enzymes were used to digest the PCR amplicons.
Results: There is an association of cytokine gene polymorphism and susceptibility to hepatitis C infection. The gene polymorphism of different genetic variants as IL10 and IL28B were the most important genetic variants. A clear difference of polymorphism found in both groups, i.e., GA polymorphism in 132 of relapser & 68 in non-responder. AA was 22 in relapser & 14 in non-responder. This is clear that gene polymorphism was more frequent in relapser
than in non-responder. These two genotypes i.e., IL10 and IL28B played important role in the eradication of HCV. Therefore, treatment needs 48 weeks rather than 24 weeks’ time, which is a normal duration of treatment.
Conclusion: This research found that gene polymorphism impart as a major contributing factor to do testing of these IL10&IL28B positions to achieve the target therapy of non-responders and relapsers (Rubi & Aslamkhan, 2016).
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