Authors: Taihua Wang, Xin Wang, Bailing Zhang, Meidai Fan, Xing Chen, Xiaohui Cui, Lina Zhao, Zhenzhen Yang, Xinyi Shi, Rongrong Li, Qingchang Fang, Xiaojuan Diao, Limin Zhang, Guoke Yang, Ying Meng, Gang Zhang.
Daily injection of insulin is a widely used treatment for human diabetes. Yet, exogenous insulin injection cannot efficiently prevent the development of iabetes complications. Recently, we reported that the transplantation of directly-generated human pluripotent stem cells (dgHPSCs) overexpressing human insulin or estrogen-related receptor γ (ERRγ) gene can not only reduce the glucose levels of human diabetes patients, but also restore the function of human pancreatic β cells, and therefore with the potential to prevent the patients from developing diabetic complications. Here, we further reported that transplantation of dgHPSCs overexpressing insulin (INS) and ERRγ genes can significantly decrease the glucose and HbA1c levels and increase the secretion of C-peptide and repair the complication of coronary heart disease in type 2 diabetes (T2D) patient. Our data proved that this strategy can secrete high level insulin in vitro, increase the secretion of C-peptide (C-P) in vivo, decrease the patient’s glucose (GLU) and HbA1c levels, and repair the patient’s diabetic complications efficiently. Thus, our report is the first to prevent and reverse human diabetic complications and with the potential to completely cure human T2D.
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