Cancer Science & Research

Abstract

KRAS Mutations: A Possible Biomarker for Advanced Prostate Cancer

Hasibe Vural, Ercan Kurar, Ebru Avcı, Canan Eroğlu, Esra Çelen, İlknur Çınar, Seda Şirin, Şahande Elagöz.

Prostate cancer is an important type of cancer in males with the highest mortality rate after the lung cancer, especially in industrialized countries. RAS oncogenes, originating from proto-oncogenes with point mutations, play an important role in cancer cell proliferation. The RAS proto-oncogene mutations occur in 25% of human cancers. The aim of the study was to investigate the potential mutation of KRAS gene at exon 2 (codons 12/13), exon 3 (codon 61) and exon 4 (codon 117/146) in Turkish patients with prostate cancer. The case group was comprised of 45 paraffin-embedded prostate cancer tissues. The control group was comprised of 20 healthy samples. The mutation analysis was performed by using PCR-based High Resolution Melting (HRM) analysis. The DNA samples of case and control groups were isolated and evaluated by HRM. Chi-square and Fisher's exact tests were used to evaluate the relationship between mutations in KRAS gene and prostate cancer. p<0.05 was considered as statistically significant. There were mutations in exon 2 (1 patient) and exon 3 (3 patients) of KRAS. In exon 4, no mutations were determined. The results of this study suggest that exon 2 and exon 3 mutations in KRAS gene may be effective in the development of prostate cancer. In addition, the results of this study may provide insight into the efficacy of anti-EGFR treatment on cases without KRAS mutation.

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