Authors: Jean-Marc N’GUESSAN, James Halbin KOUADIO, Marc ADOU, N’Guessan Georges AMANI.
Description of the Subject: The storage of fresh kola nut is a major concern in Côte d'Ivoire due to toxigenic molds proliferation.
Objectives and Methods: The aim of this study is to determine, in vitro, the antifungal effect of codified biopesticides "AG", "SI" and "TB" on Aspergillus flavus, Aspergillus niger and Penicillium sp., , Aflatoxinogenic and ochratoxinogenic molds, all by ensuring their high dose safety according to the OECD guideline 423 with Wistar rats.
Results: The results concerning the antifungal activity, showed that the mold strains are very sensitive to TB (inhibition rate; Ti > 75%) at the concentration of 7500 ppm, not very resistant or limited to SI (25% < Ti < 50%) and highly resistant to AG (Ti < 25%) even at high concentrations of 20000 ppm. Acute toxicity study results for biopesticides do not indicate any toxic effects at 2000 mg / kg of BW. However, at the dose of 5000 mg / kg of BW, only biopesticide TB was found biotolerated; AG and SI leading to toxic manifestations.
Conclusion: Taken as a whole, our results reveal that TB has a proven antifungal property and is biotolerized even at a high dose of 5000 mg / kg BW in the rat. It could therefore be recommended as a biopesticide for the conservation of kola nuts at the use rate of 7500 ppm.
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