Gamal Saadi, Iman Khaled, Abdel Aal Mohammed, Wesam Ismail, Mervat El-Ansary, Ragaa Ramadan.
Bone marrow mesenchymal stem cells (BM-MSCs) are very unique type of cells that have multipotency and immunomodulatory-regenerative properties. The aim of the present work is to ensure induction of the renal progenitor regenerative process by MSC infusion through potential upregulation of CD133 as a marker of tubular/parietal epithelial cell/ glomerular proliferation and CD44 as a marker of interstitial/ glomerular regeneration. BM-MSCs withdrawn from selected donors were isolated and further cultured to maximize their proliferation reaching 70-80% confluence. They were infused intravenously (IV) in 19 CKD patients. Renal biopsies from the 19 patients were stained for CD133 and CD44 expression before, 1 and 2 months after IV BM-MSCs injection. There was significant CD133 and CD44 upregulation in the tubulointerstitial compartment related to glomerular urinary and vascular poles as well as parietal and visceral epithelial cells following IV BM-MSCs.
In conclusion, this present work confirms the potential role of IV BM-MSCs in renal regeneration by stimulation of resident renal progenitor cell proliferation.View pdf