American Journal of Pathology & Research
Open AccessAb Initio Whole Cell Kinetic Model of Streptococcus salivarius JIM8777 (stjDNV26)
Authors: Diya Nanthakumarvani, Leesha Haarshiny Perumal, Atoshi Abirami RajKumar, Cheryl Kai Ning Kang, Sragvi Verma, Shafeeqa Abul-Hasan, Maurice HT Ling.
Abstract
Streptococcus salivarius is one of the earliest bacterial colonies of the human host which is established itself in the oral cavity within the first day after birth. It has been considered for probiotic applications due to its low virulence factor, including its potential benefits in children’s health. Mathematical kinetic models generate time-dependent profiles of simulated metabolites, which can be used to guide metabolic engineering approaches. However, there is no kinetic model of S. salivarius to-date. In this study, we present a whole cell simulatable kinetic model of S. salivarius JIM8777, stjDNV26, constructed using enzymatic reactions identified via enzymes from its published genome. The resulting model consists of 842 metabolites, 364 enzymes with corresponding transcriptions and translations, and 778 enzymatic reactions; which can be a baseline model for incorporating other cellular and growth processes, or as a system to examine cellular resource allocations necessary for engineering.
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