Introduction: Maharat Nakhon Ratchasima Hospital located in Nakhon Ratchasima Municipality, Nai Mueang Subdistrict, Mueang Nakhon Ratchasima District Nakhon Ratchasima Province Founded in 1999, is a Nakhon Ratchasima Provincial Hospital. Is the largest regional hospital in the Office of the Permanent Secretary for Health. There are a total of 1,619 beds for patients. Totally staffs are 4,669 persons with in the age between 20-60 years old. And The hospital have own Blood Bank Organization alongside and work with policy of Thai Red Cross. The blood and blood component is important for used in surgeries, blood transfusion and for clinical use. The mission of blood banks has to be the supportive threatening in hospital. By human resource of 4,699 persons; the objective of the study was to determine and evaluate the factor to affect blood donor’s behavior in Maharat Nakhon Ratchasima Hospital, Thailand.

Methods: The study is questionnaire-based and involved 369 staffs in Maharat Nakhon Ratchasima Hospital, Thailand.

Results: The data in ration of; female and male were 1.65 (268:101), used to donated blood 61.7% (228:369) a positive satisfaction level in good service 95.6% (353:369), non-remunerate or donate blood without expecting anything in return 82% (325:369).

Conclusion: Staffs in Maharat Nakhon Ratchasima Hospital have positive attitude in blood donation. And 39.1% of staffs have approach and stimulate to become register blood donor. Therefore, this research found the opportunity to get and maintain human resource in the hospital.