Synthetic DNA crown cells can be produced using sphingosine (Sph)-DNA-adenosine-monolaurin compounds and egg white. Previous experiments have demonstrated that both antibiotic compounds and antibiotic-producing cells could be produced using various combinations of DNA crown cells with partners such as microorganisms, other cells, extracts and organic compounds. However, whether these antibiotic-producing cells could be cultured has remained unclear. Previous research showed that both cell proliferation and various objects (hereafter referred to as Crown Asci-glu-glu cells nema) were observed in cultures of antibiotic-producing cells (Antibiotic crown Asci glu-glu cells) produced using egg white powder enclosing DNA (Ascidian, Ascidian sea squirt) crown cells with Glu-Glu as a partner. The present study examined whether such cell proliferation and objects (Crown E. coli-glu glu cells nema) were observed in cultures of egg white powder enclosing DNA (E. coli) crown cells with GluGlu and stimulated with GluGlu as a partner. The present findings showed that Crown E. coli-glu-glu cells nema were observed in these combinations.