The development of specific agents affecting T helper cell subpopulations has gained considerable attention in recent years, with natural products emerging as potential immune modulators for various diseases, including rheumatoid arthritis (RA). This study investigates the immunomodulatory effects of Arthrinox/BIM-A, a dietary supplement derived from five edible plants, namely black sesame, guava fruit, mangosteen aril, pennywort leaves, and soy protein, on the regulation of T helper cell subpopulations. Twelve healthy volunteers were divided into two groups, one receiving a placebo and the other taking Arthrinox/BIM-A capsules for 15 days. Blood samples were collected before and after the supplementation period, and peripheral blood mononuclear cells (PBMCs) were isolated and stimulated to assess cytokine production. In the present study, we demonstrated that Arthrinox/BIM-A can modulate the immune cell functions by reducing the production of several pro-inflammatory cytokines, including IL-2, IL-17 and TNF-α. Arthrinox/BIM-A is therefore suggested as the food supplement for balancing immunity in the management of RA patients.