Background: Lichen sclerosus (LS) presents a therapeutic challenge due to its chronic inflammatory nature, leading to sclerosis and atrophy, primarily affecting the anogenital region.

Objective: Stem cell therapy, particularly using subcutaneous adipose tissue-derived mesenchymal stem cells (ADMSCs), shows promise due to their regenerative and immunomodulatory properties.

Methods: 22 male patients with lichen sclerosus varying in degree from mild to severe were enrolled to undergo subcutaneous fat sampling using a sterile kit and subsequent processing of the stromal vascular fraction which was then freshly implanted in the same patients in an autologous manner.

Results: All patients reported a significant improvement in the signs and symptoms of the pathology already after a month and a half after therapy. Limitations: This study is limited to a small group of male patients only.

Conclusion: Our data confirm the usefulness of this regenerative adipose tissue mesenchymal stem cells autologous transplant therapy in male genital lichen sclerosus.