Non-male factor intra-cytoplasmic sperm injection (ICSI) reported decreasing implantation and live birth rates. While ICSI becomes the major insemination procedure for human assisted reproductive procedure. How to avoid the negative impact of non-male factor ICSI is the question. The hypothesis is the un-needed enzymes in acrosome area responsible for the negative impact. By selection of capacitated/acrosome reacted spermatozoa for ICSI, significant enhance fertilization, blastocyst formation, implantation and fetal heartbeat rates. The results support the hypothesis and provide a way to alleviate/avoid the negative impact of non-male factor ICSI cases.