Research Article

Molecular Characterization of Human Papillomaviruses Associated with Cervical Cancer in Brazzaville, Congo

Authors: Paola Candyse Lemba Tsimba, Luc Magloire Anicet Boumba, Fabien Gael Mouamba, DimitriMoudiongui-Mboungou Malanda, Boris Arnaud Okoko Eckouda, Jean Félix Peko.

DOI: 10.33425/2833-0390.1006

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Abstract

Knowledge of the viral ecology of human papillomaviruses is essential in the prevention of cervical cancer. This work aimed to identify human papillomavirus genotypes associated with cervical cancers in the capital city of Brazzaville. 66 paraffin blocks of cervical biopsies were collected between 2016-2017. After extraction of viral DNA, amplification and genotyping was performed using Gene Xpert technology from the Xpert HPV kit. The mean age was 54.48 ± 16.98 years (range 18-88 years). HPV viral DNA was identified in all samples analyzed, giving a prevalence of 100%. The most frequent genotype was HPV-16 (80.3%). Six percent of the patients were carriers of the HPV-18/45 combination and 12.1% of other HPV-HR genotypes. HPV-16/HPV-18/45 co-infection was observed in 1.5% of cases.

According to the type of cancer induced, HPV-16 was found in 83.3% of squamous cell carcinomas and 50% of adenocarcinomas.

In this work, the authors observed a very high frequency of HPV-16 whatever the type of cancer in Brazzaville.

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