Journal of Biotechnology and its Applications
Open AccessNovel Explants and Decontamination Protocol for In vitro Micropropagation of Red Ginger Lily (Alpinia purpurata Vieill., K. Schum) in Jamaica
Authors: Gillian ROWE, Collin SCANTLEBURY, Ryan D FRANCIS.
Abstract
The range of different explants found in the red ginger lily (Alpinia purpurata) inflorescence included miniature plantlets, corm-like propagules with leaf scars or nodal rings at the base of the mini plants, pseudo-stem regions with nodal rings, sections of the bract stalk, some with emerged axillary buds, leaf whorl tissue and leaf explants. In vitro explant tissue cultures of red ginger lily were established from four explant types on MS solid media with BAP, Kinetin and carbendazim (Methyl 2-benzimidazolecarbamate) incorporated in the media at initiation. There was no significant difference in establishment rates between different explants types nor the biocides options. Establishment ranged from 50 to 100% in two separate replications. In rooted plants after two months, despite numerically more roots, leaves and greater height in plants on carbendazim media, ANOVA showed that there was no statistically significant difference in establishment between explants with carbendazim present or absent from the media.
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