Background: Immunization is one of the most cost-effective public health interventions across countries of all income levels. The six vaccine-preventable diseases initially targeted by the Expanded Programme on Immunization (EPI) more than four decades ago laid the foundation for global immunization efforts, while the introduction of at least six additional vaccines has broadened protection and contributed to reducing morbidity and mortality throughout the lifespan.

Objective: The primary aim of this study was to assess the level of knowledge about vaccination among healthcare workers in the Central Bosnia Canton.

Methods: A total of 412 healthcare workers of both sexes, representing all age groups and professional categories employed in healthcare institutions across the Central Bosnia Canton, participated in this cross-sectional study.

Results: An intermediate level of vaccination knowledge was the most prevalent among both men (43%; n = 52/122) and women (49%; n = 142/290). The intermediate level of knowledge was also most common among healthcare workers with secondary-level professional education, representing 28.6% of the total study population. Among hospital employees, the largest proportion of respondents demonstrated an intermediate level of knowledge (54%), which was also the highest proportion compared with other healthcare institutions.