The article examines high-efficacy treatment protocols for Autism Spectrum Disorders (ASD), focusing on novel targeted therapeutic modalities within the realm of regenerative medicine. We reviewed and analyzed the implementation of advanced regenerative medicine techniques in treating ASD, emphasizing the integration of novel therapeutic modalities with biologicals targeting main pathological pathwaysof the autism. Authors discuss cellular therapies, neuropeptide interventions, therapy with precursors of neuromediators et cetera. Clinical outcomes were assessed based on improvements in behavioral, cognitive, and social functions. Patients on autism spectrum that receivedholistic biological therapy demonstrated rapid gains in self-regulation and appropriate behavior across various settings within the first weeks of the treatment. After 2-3 months, notable advancements were observed in eye contact, attention span, verbal responsiveness, and speech development. Six-month follow-ups revealed enhanced communication fluency and greater self-management. The subsequent addition of neuropeptides during the maintenance phase led to significant reductions in anxiety, aggression, and improved social skills and communication abilities. Long-term therapy showed sustained improvements in conversational ability, reduced repetitive behaviors, and enhanced social and emotional reciprocity. The integration of novel therapies with biological and neutraceuticals offers a promising approach to managing ASD, showing significant improvements in behavioral and cognitive outcomes. These findings support the potential of regenerative medicine modalities in enhancing therapeutic efficacy for ASD and warrant further investigation to optimize treatment protocols and validate long-term benefits.