Inflammantory back pain is a common symptom in Ankylosing spondylitis patients [1]. Since there is no cure for the disease, current treatment focuses on lower the symptoms and preventing progression of the disease [2]. Many laboratories had tried to use stem cell to teat Ankylosing spondylitis related symptoms and receive positive response from patients. Here we used SB cells® to treat Ankylosing spondylitis patients. Not only patients showed lower pain symptom, but also patients did not report any side effect from the treatment. Here we used the SB cells® which purified from patients’ own body in their stem cell treatment. With our initial success, we are planning to perform SB cells® treatment on a larger scale of Ankylosing spondylitis patients in the hope of obtaining larger data volume.