Today, one of the approved methods is mostly used in measuring diseases, especially in acute lymphatic Leukemia (ALL) Is the measurable residual diseases (MRD) strategy technique. MRD adverts to a population of leukemia cells in the bone marrow and, less usually in the peripheral circulation after treatment. This strategy was applied by association of several techniques and methods that utilized the patients’ samples mostly treatments. In this review consist the types of methods which are recently used in MRD such as types of Next-generation sequencing (NGS.) And types of flow cytometry (FC). Moreover, the notable points in this review article are to highlight the problems of MRD measuring and suggest the ways that solve the problems. The results of this review modified two types of challenges which one of them depend on sensitivity and specificity of measuring in methods, procedures, and technologies and the second one depends on the changeable factors of analysis which a cure in relapse of diseases after treatment in ALL.