HbA2 determination plays a key role in screening programs for hemoglobinopathy and reducing diagnosis mistakes. The normality area for Hb A2 is often wide. It is advisable for each laboratory to validate the State of Hb A2 for local population because low values with microcytosis can indicate deficiency anemia or alpha-thalassemia. However, high level is for beta-thalassemia or other hemoglobin variant. We have performed a prospective study in search of local reference values for hemoglobin A2 and F. We have used the most powerful methods for hemoglobin fractions analysis: Capillary electrophoresis and HPLC. Five hundred healthy transfusion center’s donor interviewed, selected and collected for current study. Genetic analysis show no mutation in alpha and beta-globin gene. Exclusion and inclusion criteria well established and respected. Significant correlation detected (p<0.05) between capillary electrophoresis and HPLC dual program A2/F (D10) after outlier’s exclusion. Local reference limit found here correlate well with interval confidence of sebia and biorad.