DNA crown cells can be prepared using sphingosine (Sph)-DNA-adenosine-monolaurin compounds and egg white. Numerous types of DNA crown cells have been prepared to date. In previous experiments, it was demonstrated that antibiotic-producing cells were isolated from beer that was inoculated with DNA (Streptomyces) crown cells and yeast, enclosed in egg white powder, DNA (Streptomyces) crown cells and yeast, or DNA (HepG2) crown cells and yeast. Moreover, antibiotic-producing DNA crown cells could be produced using beef extract instead of yeast. The present study examined whether antibiotics or antibiotic-producing cells could be produced using salmon roe, a type of animal cell. The results showed that antibiotic-producing cells could be produced using salmon roe in combination with DNA (HepG2) crown cells. The resulting cells were named antibiotic-producing Crown-HepG2-Salmon Roe-cells.