Background: Hepatitis C Virus is a major human pathogen infecting lots of individuals worldwide with increasing risks of chronic liver diseases. Hepatitis C viral infection affects people of all ages, races and gender causing both acute and chronic inflammation of the liver. It is responsible for most cases of liver transplantation in US and Britain. Blood testing is one of the reliable methods in use to diagnose Hepatitis C virus to detect the presence of antibodies to HCV, using an enzyme immunoassay.

Aim: Was to determine the prevalence of Hepatitis C Virus among patient and voluntary blood donors who presented at David Umahi Federal University Teaching Hospital Uburu in Ohaozara LGA, Ebonyi State. Methodology: Blood samples were appropriately collected from the subjects and screened for Hepatitis C Virus infection using the antibody (Ab) rapid test kit (an enzyme immunoassay technique).

Result: A total of 93 patients and blood donors were tested for hepatitis C virus infection, comprising of 52 (55.9%) female and of 41 (44.1%) male gender. Out of 93 patients tested over the period of 6 months, 2 (2.2%) were hepatitis C virus positive giving a period Sero-prevalence rate of 2.2% with no significant difference in prevalence among gender and age of participants.

Conclusion: There is urgent need to take public health measures seriosly to reduce disease burden and transmission, by routine screening of all for HCV infections and health education campaigns to people on the dangers associated with HCV infection.