Dry eye syndrome (DES) has become increasingly prevalent due to the prolonged use of digital devices in modern society. Individual supplementation with lutein, anthocyanins or astaxanthin demonstrated potential benefits for eye health. This study aimed to investigate the effects of lutein complex supplementation on DES, visual function, and serum lutein level in healthy individuals. Twenty-four participants, aged 20~60 years, received lutein complex supplements (containing 10 mg lutein, 2 mg zeaxanthin, 10 mg anthocyanins, 75 mg proanthocyanins, and 1 mg astaxanthin) for 12 weeks. Ophthalmological examinations and blood test were carried out, and a questionnaire was administered at weeks 0, 6, and 12 of the study. After 12 weeks of the intervention, the serum lutein level had significantly increased, and intraocular pressure (IOP) had significantly decreased. It also improved symptoms of eye strain, heavy eyelids, dry eyes, eyes itching, photophobia, blurred vision, sore eyes, blurred vision at night. This suggests that lutein complex supplementation may increase the serum lutein level, reduce the IOP, and offer potential benefits for DES.